Review Session Details
- Understanding general ligand binding (i.e. formulate equation and associate it to Kd)
- Learn to manipulate the simple Kd equation into complex ones
- Graphically understand how protein-ligand system is interpreted
- Cooperativity in ligand binding (i.e. Hill plot)
- Assess the two types of cooperativity models (MWC vs. KNF model)
- Apply protein-ligand cooperativity to oxygen transport in the body
- Understand enzyme catalysis graphically and in-terms of Gibbs free energy
- Understanding the Briggs-Haldane model and the steady-state approximation
- Measuring the rate of enzymatic reactions with Michaelis-Menten enzyme kinetics
- Understand the Michaelis-Menten equation
- Graphically understand how to interpret Michaelis-Menten enzyme kinetics
- Graphically understand the lineweaver-burke plot (double reciprocal of Michaelis-Menten graph)
- Fully understand the following kinetic parameters: KM, Kd, kcat, kcat/KM
