BCH2333/2733: Protein Binding and MM Kinetics

BCH2333/2733: Protein Binding and MM Kinetics

Review Session Details
  • Understanding general ligand binding (i.e. formulate equation and associate it to Kd)
  • Learn to manipulate the simple Kd equation into complex ones
  • Graphically understand how protein-ligand system is interpreted
  • Cooperativity in ligand binding (i.e. Hill plot)
  • Assess the two types of cooperativity models (MWC vs. KNF model)
  • Apply protein-ligand cooperativity to oxygen transport in the body
  • Understand enzyme catalysis graphically and in-terms of Gibbs free energy
  • Understanding the Briggs-Haldane model and the steady-state approximation
  • Measuring the rate of enzymatic reactions with Michaelis-Menten enzyme kinetics
  • Understand the Michaelis-Menten equation
  • Graphically understand how to interpret Michaelis-Menten enzyme kinetics
  • Graphically understand the lineweaver-burke plot (double reciprocal of Michaelis-Menten graph)
  • Fully understand the following kinetic parameters: KM, Kd, kcat, kcat/KM