BCH2333/2733: Protein Binding, Michaelis-Menten Kinetics

BCH2333/2733: Protein Binding, Michaelis-Menten Kinetics

Review Session Details
  • Kozlowski: Lectures W7 and W8A
  • Boddy: Quiz 4
  • Protein-Ligand binding (i.e., Kd)
  • Saturation/Binding curves
  • Cooperativity and Hill plots
  • Sequential vs. Symmetry┬ámodels
  • Cooperativity of oxygen-hemoglobin transport
  • Enzyme catalysisand free energy
  • Steady-state approximation
  • Michaelis-Menten enzyme kinetics (i.e., Km and Vmax)
  • Michaelis-Menten equation and plots
  • Lineweaver-burke plots (double reciprocal of Michaelis-Menten graph)
  • KM, Kd, kcat, kcat/KM
  • Review Session will include problem solving from past Midterm questions.
  • All registered students will receive a "Midterm Package #4" that includes multiple Midterms/Quizes related to the above topics from previous years given by uOttawa Professors.