BCH2333/2733: Midterm 2: DNA Structure and Lipids

BCH2333/2733: Midterm 2: DNA Structure and Lipids

Review Session Details
  • Review primary DNA structure (i.e. base tautomerization, classic base pair H-bonding, c2/c3 endo sugar rings)
  • Understand the inter- and intramolecular interaction in a DNA double helix
  • Analyse and understand DNA secondary structures (i.e. DNA helices: A, B, and Z)
  • Remembering Chargaff's Rule
  • Understand properties of triple DNA helices (H-DNA)
  • Palindromes and formation of hairpins and
  • DNA denaturation and melting curves
  • Types of lipids and their structures
  • Lipid membrane properties (i.e. fluidity, lateral and transverse diffusion, and cholesterol)
  • Fatty acid nomenclature
  • Understanding general ligand binding (i.e. formulate equation and associate it to Kd)
  • Learn to manipulate the simple Kd equation into complex ones
  • Graphically understand how protein-ligand system is interpreted
  • Cooperativity in ligand binding (i.e. Hill plot)
  • Assess the two types of cooperativity models (MWC vs. KNF model)
  • Apply protein-ligand cooperativity to oxygen transport in the body