Review Session Details
- Understand the functions of polymerase I and III in E. coli
- Learn the steps of "nick translation" by polymerase I
- DNA ligase activity and mechanism
- DNA repair mechanisms (mismatch repair, base/nucleotide excision repair, direct repair)
- Understand the key enzymes involved in DNA repair and their function
- Mismatch repair in E. coli (via MutS, MutL and MutH)
- Direct repair via methylation of guanine
- Overview of transcription and roles of key enzymes/regions
- Function of transcription bubble
- Transcriptional regulation in prokaryotes (lac operon)
- Drawing D and L amino acids
- Finding overall charge and pI of amino acid or peptide
- Properties and structure of amino acids
- Drawing polypeptides
